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Of the sequenced ESTs that responded significantly, 118 genes were successfully annotated; 170 matched unnamed or hypothetical proteins, or did not match the described annotation selection criteria; i.e. BLASTx searches resulted in expect-values greater than 1 × 10-5 and scores below 50.
Further, for U95 data, a previously described annotation and cross reference tool (Chipcomparer) that facilitates for comparison across Affymetrix platforms (U95 and U133) by matching corresponding probeids and relative expression values was employed.
Functional annotations were assigned to all unique transcript sequences using a previously described annotation pipeline [ 28].
SAGE libraries were annotated using SAGEmap (http://www.ncbi.nlm.nih.gov/projects/SAGE/). Mitochondrial tags were identified using previously described annotation[ 16].
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Starting from a common VCF file and after selecting for significant results from company reports, we find no overlap among described annotations.
The Annotator identifies ontology terms in submitted text and returns formally described annotations in the form of a Uniform Resource Identifier.
Alnazzawi et al. describe annotation of a corpus aimed at encoding detailed phenotypic information.
Additionally, the results of these searches were manually examined in order to assign the best describing annotation to EST clusters.
In addition to training sessions, we provided a downloadable tutorial describing annotation approaches that integrated the use of BGD, Ensembl and NCBI.
Once the variants were called, the previously described Cassandra annotation suite was used to annotate the identified variants.
Subsequently, the 13 kDa Psb28 photosystem II protein was incorrectly described during annotation of the Arabidopsis thaliana nuclear genome as a PsbW-like protein.
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