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Minimal medium was prepared as previously described, adjusted to pH 7 with sterile solutions of HCl and NaOH, and 0.5% (vol./vol). 2 µM α-tocopherol acetate (vitamin E acetate) was added to the medium.
Working bacteria culture was prepared as previously described, adjusted to be equal to 0.5 McFarland standard (1 × 10 cfu/ml) and further diluted by mixing 1 part of adjusted culture with 199 parts of TS broth to meet 5 × 10 cfu/ml.
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Overall, 28 trials described adjusted analyses in the protocol (n=18) or publication (18).
Some described adjusting the intensity of sports activities and avoiding social activities.
Murine peritoneal macrophages cells were isolated as previously described and adjusted to 4 × 106 CFU/mL (Guan et al. 2015; Zhang et al. 2008).
All the standard errors of the estimated parameters obtained by the previously described analyses were adjusted to take into account that the repeated measurements at different times for the same patient were clustered within the patient [ 11].
Spermatozoa collected as described above were adjusted to 2×106 cells/ml in BWW and incubated for 2 and 4 h.
The initial pH of both media described above was adjusted to 7.0.
Data analyses were performed as described below but adjusted to analyze a simpler experiment.
Average sarcomere length was determined by means of a spatial Fourier transform as described previously and adjusted to approximately 2.2 µm [ 6].
While only metal nanowires fabricated on Si and glass substrate are shown in this letter, the approach described here can be adjusted to deposit any conductive materials, oxides, conductive polymers and semiconductors, on any solid substrate as well as flexible polymer substrate.
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