Colonies were tested as described above using primer sets 5′selD and 3′selD.
cDNA synthesis was performed as described above, using as much total RNA as we could extract.
Real-time qPCR was performed as described above using β-tubulin mRNA as a loading control.
BaBar have performed a similar Dalitz plot analysis to that described above using neutral B decays.
Samples were then immunoblotted as described above using a DNP antibody (1 150) provided with the OxyBlot kit.
(b) Immunoblot analyses of the samples described above using antibodies against specific subunits of Integrator and Mediator.
We generated two synthetic datasets starting from the E. coli regulondb network and yeast MacIsaac network (both described above) using GeneNetWeaver.
Assembly of aptamer nanostructures in cell-binding buffer was performed as described above (using 1.5 µM C10.36 or Waz and 0.5 µM 3WJdB or AF488-anti-tail).
All SFA imaging experiments were performed simultaneously with CARS imaging on the same system as described above, using the same wavelengths and power levels.
The enzyme activity was determined under the condition described above using NADH as coenzyme.
Forbes researcher Paul M. Murdock compiled Barack Obama's positions, described above, using material from the candidate's public statements, news reports, voting record, Web site and other sources.
