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Due to the high degree of homozygosity we stopped inbreeding the lines developed from MT collections after only two single seed descent generations under laboratory conditions.
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Three 48-plex GBS libraries were constructed from a set of 138 progenies from the F11 single-seed descent generation, along with replicated samples of each parent, respectively.
Further, the marker density on the consensus map is suitable for whole genome pedigree analysis, and calculating identity-by-descent through generations.
The process of evolution has been identified as (i) any directional change; unfolding; (ii) any cumulative change in the characteristics of organisms or populations from generation to generation; descent or development with modification; evolve (Lincoln et al., 1998).
The RILs (n = 177) were derived from the F2 generation by self-fertilization and single-seed descent for five generations, creating the F6 RIL mapping population that we used.
But Trujillo himself had a grandmother of Haitian descent, as had generations of both Dominican elites and working people.
For each accession, the seeds were produced by single seed descent over two generations in a Cirad greenhouse in Montpellier to ensure that the samples were homogeneous.
Descent from four generations of Baptist ministers--from her great-grandfather to her brother, perhaps accounts for her preaching tone toward the disadvantaged.
All the study participants were of the same Caucasian (Spanish) descent for ≥3 generations.
Young leaf material from 3-week-old Brachypodium Bd-21 plants derived by single-seed descent for 9 generations was flash frozen in liquid N2 and stored at −80°C for nuclei isolation.
Plants were subjected to single-seed descent for four generations to increase their genetic homogeneity.
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