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Then they were de-paraffinised xylene twice and rehydrated in descending concentration of ethanol.
(3) The reaction rates of coal combustion and gasification fell for descending concentration of gasification agents absorbed on coal surfaces of the cavity wall.
Embryos were washed in descending concentration of 0.2x SSC in PBST solution.
Sections were passed through a series of ethanol solutions of descending concentration [3 min in each of 100, 95 and 70% (v/v) ethanol in water], and stained for 5 min with Cresyl Violet [0.05% (w/v) aqueous Cresyl Violet, 2 m m acetic acid and 5 m m formic acid in water].
For cresyl violet staining, mounted and air-dried sections were rehydrated in serial alcohol dilutions with descending concentration and stained with 0.1% cresyl violet (Sigma, Swindon, UK) in H2O for 10 min. Sections were rinsed in H2O and dehydrated through serial alcohol dilutions with ascending concentration and cleared with xylene and histoclear (Sigma).
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Samples were assessed for total AO activity using the peroxyl radical scavenging capacity assay where each sample was diluted to 5 descending concentrations, plated in triplicate.
Following incubation of tissue samples with xylol and descending concentrations of ethanol, antigens were retrieved using citrate buffer, pH 6.0 (DAKO, Glostrup, Denmark) for 30 min at 98°C.
Paraffin sections were dewaxed in xylene and rehydrated in descending concentrations of alcohol.
Briefly, 6 µm thick sections of normal and diabetic rat pancreas were deparaffinized with xylene, and rehydrated with descending concentrations of ethanol.
Histological slides were deparaffinized, rehydrated in descending concentrations of ethanol, and incubated with 4',6-diamidino-2-phenylindole (DAPI, Invitrogen, Carlsbad, CA,) for nuclear counterstaining.
For quantitative studies in AMI paraffin embedded tissue sections (4 µm thick) were mounted on microscope slides and deparaffinized for 10 minutes in xylene at room temperature and dehydrated through descending concentrations of ethanol.
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