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Secondly, in positive samples in both groups there was a significant decrease in staining intensity with increasing dermal depth (Table 2; Fig. 2).
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However, the structures of the human dermis are not homogeneous in terms of either interstitial spaces or architecture at different dermal depths.
Mice treated with hADSCs and VEGF-C hydrogel showed a significantly decreased dermal edema depth compared to the groups of mice that received hADSCs only or VEGF-C hydrogel only.
The dermal wound depth and width were the smallest in the ovx+YCJ group, as compared to the other groups following a 14-day-treatment period (p < 0.01).
At this depth, dermal papillae dominate the images and their density is high.
TransCyte™ is another type of skin substitute that is used in the treatment of mid-dermal to indeterminate depth burn wounds that typically require debridement and that may be expected to heal without autografting.
The depth of dermal injury can vary within this group, with wounds of greater depth requiring longer time to heal.
Tumour thickness, depth of dermal penetration, and the degree of cell atypia were found by the authors to be univariate predictors of outcome (Kruskal-Wallis test P<0.01).
The median skin thickness decreased with treatment from 2.23 mm (IQR 2.0 2.95) to 2.0 mm (1.81 2.36) as measured from the granular cell layer to the dermal subcutaneous interface or the greatest depth on H&E (p<0.01).
The samples were embedded vertically so that the sections went through epidermis and dermis to a depth of 1500 μm from epidermal dermal junction and thin 60- to 70-nm sections were mounted on Formvar-coated (Sigma-Aldrich, St . Louis MO, USA) 50- or 100-mesh grids and stained with uranyl acetate (2.5% in 50% ethanol) and lead citrate (3%).
This process was applied to all the A-scans of the OCT volume, and two en-face slope maps were obtained; papillary-dermal birefringence (PDB) map obtained at the 100-200µm depth region from the skin surface and reticular-dermal birefringence (RDB) map obtained at the 200-300µm depth region.
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