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For the expression analysis we used COS-1 cells (transformed green African monkey kidney cells) that can be transformed with the Listeria as DNA carrier at high rates [ 22], B16 melanoma cells deriving from a fast growing murine melanoma, and 4T1 cells deriving from a stage 4 breast cancer.
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Our group conducted a differential high-throughput drug screen in gastric cancer cells derived from a stage IV HDGC patient with a truncating c.1380delA CDH1 germline mutation and gastric cancer cells derived from a liver lesion of a gastric cancer patient with wild type CDH1 [12].
IGR-N91 cell line was derived from a stage 4 NB infiltrated bone marrow [20].
COGA-13 cells, which were derived from a stage pT2 carcinoma graded G3, exhibit only weak alkaline phosphatase activity (Table 1).
The EOC cell lines were derived from a stage III/grade 3 clear cell carcinoma (TOV-21G), a stage IIIc/grade 3 endometrioid carcinoma (TOV-112D), and from the ascites fluid of a stage IIIc/grade 3 adenocarcinoma (OV-90), all from chemotherapy naïve patients, as described [ 2].
However, in the same study CAV1 expression in the highly differentiated Ishikawa cell line was significantly lower than in CHEC cells derived from a stage I grade III endometrial adenocarcinoma, underscoring that the relationship between CAV1 expression and tumor progression is complex.
The framework is derived from a staged model of trust and allows predictions to be made concerning user engagement with different health websites.
Reduced-order nonlinear models are derived from a stage-by-stage balance model by partitioning the column into distinct compartments and applying time-scale arguments to convert most of the ordinary differential equations (ODE) describing each compartment into simpler algebraic equations.
Others, proponents of the "Slow boat" model, used a genetic approach, and showed that most Polynesian lineages derived from a staging post in Wallacea (West Indonesia), pre-established in the early Holocene or before [41].
The outline, as the term implies, gives the essence of the action or story and may present either an original idea or, more usually, one derived from a successful stage play or novel.
However, our current analysis is limited to HCT116 cells that are derived from a late stage colorectal carcinoma.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com