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From the reported neutralizing data, we derived the consensus sequence for B-cell epitope ILNCNDSLGIALFYKCW and experimentally verified E2 neutralizing motif I-L-N-C-[NQ]-[DE]-S-L-G-[IL]-A-L-F-Y-[KNRS]-C-W.
We derived the consensus sequences from position-specific scoring matrices (PSSM, also known as scoring profiles) generated by iterative PSI-BLAST ('blastpgp') (Altschul et al., 1997) searches of the redundancy-reduced UniProt (Apweiler et al., 2004) database containing about 1.5 million sequences.
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For deriving the consensus sequence using arrays with 1176 substrates, we considered only positions −3, −2, −1, 0, 1, 2 & 3 because not all peptides were 9 amino acids in length and also while aligning them based on a fixed central phosphorylation site, we did not have an equal distribution of amino acids at the −4 and +4 positions.
Each group of sequences was combined to derive the consensus sequences.
The sequences were subsequently used to derive the consensus binding site for the IRF6-DBD (Fig. 2C).
A common remedy is the selection of the most frequent nucleotide at each alignment position to derive the consensus sequence.
For nucleotide sequence analysis of the intermediate repeats in the msh1 mutants, we used a custom-made script to derive the consensus sequence.
Projection projects all l-mers from the input sequences onto many buckets by hashing and then derives the consensus sequences to select some valid buckets.
Then, multiple sequence alignment is performed for each contig to derive the consensus sequence, an approximation to a target genome fragment.
However, the objective of GSEA and PAGE was to gain insights into biological mechanisms by clustering genes across studies, while our focus was in deriving the consensus information along with a reliability score.
A single stochastic mapping was done per tree using the last 2,000 post-burnin trees of the 20,000 trees used to derive the consensus tree of Fig. 2 and the ancestral states were inferred for the consensus tree from the MrBayes analysis.
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