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Both 1Dx2s and 1Dx2f subunits were simultaneously expressed in the derived line, suggesting that one x-type gene duplication event at the Glu-D1 locus occurred.
The MABS derived line 'WA8059' also exhibited more uniform kernel weight as evidenced by a significantly lower kernel weight standard deviation (Figure 3b).
Without the MABS, 79% of the donor chromosome 1B (carrier chromosome) was retained in the BC4F7 derived line 'WA8046' (Figure 3a).
The MABS derived line 'WA8059' was compared with a BC4F7 line 'WA8046' that was developed using the same two parents, but by performing backcross breeding with phenotypic selection but without MABS.
New information would benefit also future morphology-based phylogenetic analyses, the result of which, due to different characters and character state scoring, have so far yielded contrasting results with regard to the position of Oregodasys: e.g. a basal position in Hochberg and Litvaitis [18], more derived line in Hochberg & Litvaitis [30] and in Kieneke et al [19].
WT or G93A SOD1 expressing NSC-34 cells were obtained by stably transfecting a NSC-34 derived line expressing the tetracycline-controlled transactivator protein tTA with pBI-EGFP containing WT or G93A hSOD1 cDNA cloned downstream to the tetracycline-responsive bidirectional promoter [67] and express similar amount of human and murine SOD1 (Figure S5).
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With federal money banned from being used in any part of the work on the derived lines, researchers had to divide their laboratories and go to extreme lengths to separate research materials based on the financing source.
Subsequently, four of the ex vivo derived lines were analysed for corresponding miRNA expression.
The current NIH guidelines allow research with newly derived lines, which Lamberth sees as inseparable from the destruction of embryos.
At least two independently derived lines on the autosomes were tested for each construct.
The artificially selected Agg line represents an extreme phenotypes beyond that seen in any of the naturally derived lines.
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