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end{aligned} (47 Now, the formula of (lambda _mathrm{s}^epsilon) is derived from the normalization condition, (int _{-infty }^infty |psi _{mathrm{s},n}^{epsilon }( {q},t)|^2 dq =1).
The following analyses were performed on gray matter segments that were multiplied by the non-linear components derived from the normalization matrix (modulated gray matter volumes).
Normalized grey matter segments were non-linearly modulated; i.e. they were multiplied by the Jacobian determinant of the non-linear deformations derived from the normalization matrix.
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The sequencing results from the sequences derived from the normalized library revealed many transcripts unrelated to the seed storage proteins, confirming the effectiveness of normalization (Additional File 1).
Spatially normalized images were modulated by the Jacobian determinants derived from the spatial normalization to ensure that the overall amount of each tissue class was not altered by the spatial normalization procedure.
Modulation was achieved by multiplying voxel values in the segmented images by the Jacobian determinants derived from the spatial normalization step.
While introducing the Jacobian determinants derived from the spatial normalization, the non-linear spatial transformations modulate the regional differences of partitioned GM/WM from relative (concentration) into an absolute amount (volume) [23].
White and grey matter voxel values were multiplied by the Jacobian determinants derived from the spatial normalization step (Jacobian modulation) to preserve the initial volumes, to obtain modulated white matter images.
The normalized, segmented gray and white matter images were then modulated by calculating the Jacobian determinants, derived from the special normalization step, and multiplying each voxel by the relative change in volume, as in the method of Good et al. (2001) [28].
However, we report the results derived from the lowess normalization as it produced the best match to the NGS data.
Then, to modulate the GM and WM tissue for group volume comparisons, the nonlinear deformation parameters derived from the previous normalization procedure were used.
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