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The baseline level of proHGF and HGF derived from the medium and/or endogenous proHGF and HGF were firstly detected by an ELISA assay, in order to establish that they would not affect the measurement of mESCs pluripotency and differentiation in our assay (Fig. S3).
This provides evidence that the incorporated inositol is exclusively derived from the medium.
In the cultured cells, exogenous fatty acids are exclusively derived from the medium and AA might be enriched compared with DHA, as it is also the main fatty acid species in PE under healthy conditions (Fig. 4C).
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The medium composition (Table 1) is derived from the CDM medium [ 17, 18] with a ten-fold reduction of Branched-Chain Amino Acid (BCAA) concentrations i.e., valine, leucine, isoleucine.
We also investigate the molecular identity of the metabolites derived from the conditioned medium.
It is a concern that non-physiological lipids derived from the culture medium used to grow the human lymphoblastoid cells may load into the secreted CD1d molecules and thus become part of the ligand pool analyzed here.
Occasionally, virus-like particles were demonstrated by electron microscope in material derived from the culture medium.
Its substrates were derived from the BIOLOG medium experiments http://www.biolog.com.
Hence, the blastosol can provide a highly purified sample of embryo secretions, free from contaminants derived from the culture medium.
The extract contains various types of constituents, such as polysaccharides, including β-glucans, triterpenes, and lignin derived from the culture medium and its digestion products by the mycelia.
According to Diaz et al. [ 32] even for identical lipases produced by different methods of cultivation (submerged and solid-state), there may be thermostability differences caused by the binding of nonprotein compounds derived from the culture medium through noncovalent bonds to the lipases, changing their physical and chemical properties.
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