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The above observations and predictions derived from the constructed host-pathogen interspecies interaction networks allowed us to explore the dynamics and underlying mechanisms by which C. albicans interacts with zebrafish during infection, providing new insights for future experiments in infectious diseases.
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Increasing concentration of purified his-tagged PTB protein (500 and 800 ng) was incubated with 5 pmoles of in vitro transcribed RNA corresponding to the IRF2 bicistronic RNA (derived from the construct pRIRF2F) and binding reaction was performed in a final volume of 20 µl at 30°C for 20 minutes.
Protein samples derived from the construct were crystallized into two different crystal forms, both of which contained four molecules per asymmetric unit.
The psychological variables used in this study were derived from the constructs of the Health Belief Model [ 28] and the Theory of Planned Behavior [ 29].
Three different types of bias are distinguished, depending on whether the source of interpretation problems derives from the construct, method of the study, or specific items (called construct, method, and item bias or differential item functioning, respectively).
Most of the interview questions were derived from the framework constructed by Siyavora (2010), amended to fit the purpose of this study, with some supplementary questions generated by the researcher for the particular research context of CCs in Jordan.
All other NEMO constructs were derived from the NEMOw construct using site-directed mutagenesis (QuikChange XL II mutagenesis kit) and expressed and purified in the same manner.
Control eGFP expressing vectors were derived from the pCCL.PPT.hPGK.eGFP construct (in place of the TCR containing construct) in a similar manner.
Unexpectedly, a heterologous portion of the crystallized complex derived from the expression construct mimics an SBM, and binds Smt3p in a manner resembling SBM binding to human SUMO family members.
The viral copy numbers were calculated according to the standard curve derived from the ORFK9 construct (Figure 4B).
This band was absent in medium samples from untransfected MSU-1.1 cells, confirming that it is derived from the recombinant construct.
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