Primary cultures were derived from specimens selected on the basis of the disease course before treatment of the patient (e.g. no chemotherapy).
Gut tissue samples were derived from surgical specimens of patients with established adenocarcinoma of the colon (Department of Surgery, University Hospital Heidelberg; informed consent was given by the patients).
The xenografts used in this study were derived from diagnosis specimens of T-ALL patients, two of whom relapsed early (<1 year) and succumbed to their disease (ALL-27 and ALL-31, Table 1).
We have demonstrated that the BrdUrd comet assay is suitable for the measurement of DNA maturation and integrity in very small numbers of primary epithelial cells derived from biopsy specimens of human colonic mucosa.
Primary cell cultures were derived from biopsy specimens of medulloblastoma patients under a protocol approved by the Institutional Review Board at the University of Iowa Hospitals and Clinics.
For CC, PDXs were generated from tumour tissue derived from surgical specimens of patients with colorectal cancer.
We used 3 ALL xenograft clones derived from spleen specimens of xenografted NOD/SCID mice inoculated with leukemia cells from pediatric BPL patients.
In this study, we have analysed the dynamics of chromosomal instability and chemosensitivity during glioblastoma progression using a unique cell model comprising three cell lines derived from surgery specimens of a long-term surviving glioblastoma patient.
The gliomas named as 'HTZ' were primary tumour cell cultures derived from surgical specimens of human high-grade gliomas (WHO Grade IV) as described before (Bogdahn et al, 1989).
Primary leukemia cells from 17 BPL patients and 10 BPL xenograft clones derived from spleen specimens of xenografted NS mice inoculated with primary leukemia cells from 5 pediatric BPL patients (4 in relapse, 1 newly diagnosed) were used in the described experiments.
Both H2 and LA-1 strains were derived from fecal specimens of HA patients in China and were isolated in cell cultures., For preparation of the H2 vaccine, the original wild virus strain was passaged in new monkey kidney (NMK) cells 15 times at 35 °C, 5 times at 32 °C, and passaged in human diploid fibroblasts (KMB17) 4 times at 32 °C.
