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In fact, the two climate-change scenarios used in the assessment are derived from models selected by the United Nations-affiliated Intergonernmental Panel on Climate Change.
Thus, acquired methylation in the SVHUC cell line could be derived from repeated passages and selection during cell culture.
All cell lines derived from in vivo passages are summarized in Table S1.
As a species-specific target antigen, we used L. infantum promastigotes derived from various culture passages.
In the samples derived from all 5 passages, the virus titer (CCID50/mL) was determined by a virus titration assay.
All the infused UC MSCs were derived from passages 2 to 4, with rigorous purification and quality control.
Virus strains derived from passage are denoted as either mosquito-passaged (SLEV MP) or chicken-passaged (SLEV CP) together with the number of passages completed.
M006x is a cell line derived from M006 passage by through a mouse as a tumour xenograft.
Each biological replicate consisted of fibroblasts derived from a randomly selected fetus and cultured for two passages.
Interestingly, we see a loss of the claudin-3 protein in hCMEC/D3 cells from passage 31 (lane 2) compared to cells derived from passage 26 (lane 3).
Of the B. garinii genome we sequenced equal amounts of clones from high and low passage strains; of the B. afzelii genome only one third of all sequences were derived from the low passage strain.
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