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pBAC-BoHV-4ΔIE1 was stable as observed by the absence of differences among the restriction profile of pBAC-BoHV-4ΔIE1 derived from different passages (Fig. 1c).
For each cell line a pool of RNA derived from different passages was obtained, and samples were processed according to the experimental procedures specified by Affymetrix®.
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The strains Rlow and Rhigh derive from different passages of strain R grown in artificial medium [ 14, 15].
Two influenza A viruses of different subtypes, derived from different species and showing no pathogenicity toward mice, were forced to evolve by serial passaging in mouse lungs.
Their sense of authority is derived from different values and experiences.
The storm event on May 8, 2005, was associated with the passage of a CIR. Figure 2 describes time series of the Dst index and the Kp index, as well as the trough position derived from different satellites for this storm.
The samples were derived from different animals.
Importantly, the trend of the intense ACE2 expression of 2B4 cells appeared to be stable, as cells derived from two different passages (i.e., #6 and #12) exhibited little difference, if any, in the expression of ACE2 protein.
We extracted total RNA from 7 cultured MSC samples deriving from different mice and passages (see Methods) with proven immunomodulatory ability, and analyzed their gene expression profiles by hybridization onto Affymetrix Mouse Genome 430 2.0 arrays.
The data were acquired from three independent experiments (conducted in triplicate) using cells from different passages.
There was no variation between the phenotypes of cells from different passages or freezing thawing cycles.
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