The pVHL defective cell line RCC10 [ 26], derived from a sporadic renal clear cell cancer, was used as a positive control for BNIP3 expression, and a pVHL wild type cell line (20) and a slide without the denatured probe were used as negative controls.
TOV21G was derived from a patient with a clear cell carcinoma, a disease which exhibits distinct clinical course that differs from HGSC, HGEC and undifferentiated adenocarcinomas, cancer types from which the other EOC cell lines were derived (see Table 1) [ 19].
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The EOC cell lines were derived from a stage III/grade 3 clear cell carcinoma (TOV-21G), a stage IIIc/grade 3 endometrioid carcinoma (TOV-112D), and from the ascites fluid of a stage IIIc/grade 3 adenocarcinoma (OV-90), all from chemotherapy naïve patients, as described [ 2].
The RCC cell line ClearCa-5 was derived from a typical representative of the clear-cell type of RCC (Wethkamp et al, 2006).
The VHL defective cell lines 786-O (originally from ATCC), A498 (originally from ECACC) and RCC4 (a gift from CHMC Buys) were all derived from renal clear cell tumours.
The HRTC cultures were derived from RCC clear cell (4 cases) or chromophobe (2 cases) subtypes.
ES-2 which is derived from ovarian clear cell carcinoma [27] had the common heterozygous p.V600E missense mutation.
However, the present study uses the original terminology, coagulopathy of trauma and ACOTS as a representative nomenclature for the various terminologies derived from ACOTS without a clear definition.
