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For all PglA derived constructs, an apparent pH optimum between pH 5.5 and 6.5 was observed.
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The respective 97-mer oligonucleotides (IDT, see sequences below; guide sequences are marked in bold) were cloned into the optimized miR-E backbone of RT3GEN (Fellmann et al, 2013) or into a derived construct expressing a blasticidin resistance cassette instead of Neo (RT3GEB).
To directly assess genetic regulators of derived constructs, we performed a principal components analysis of behavioral measures from each of the tests, excluding those that were arithmetically derived from one another, and individual time points from sets of repeated measures.
Moreover to date the literature has not adequately addressed possible mechanisms of change inherent to MI-approaches using theoretically derived constructs [ 53, 54] and lacks a more fine-graded description of specific subgroups that benefit from such an approach (i.e., moderators).
coli shuttle vector pTSSCm33 or derived constructs were selected and routinely grown using 10 mg/l tetracycline in the growth medium.
E. coli V330 and V66 strains were transformed with the pGL10 derived constructs expressing different recBCD alleles for the phage assays.
Histology revealed a collagenous extracellular matrix and the contractile responses measured for these vessels were stronger than dermal fibroblasts derived constructs although weaker than SMCs-derived constructs.
All inserted fragments in the derived constructs were commercially sequenced in Beijing Genomic Institute (BGI, Beijing, China).
Familism and parental respect are culturally derived constructs rooted in Hispanic and Asian cultures, respectively.
In fact, the link between the hypothesised and derived constructs is clear.
All pEnh-Reentry derived constructs were brought into the ap locus by ΦC31-integrase mediated recombination (see Figure 4, D and E).
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