To derive closely paired in vitro data from these litters of ΔF-CFTR mice, tracheae were excised from these same mice in which CFTR NPD measurements were performed previously to isolate and establish mouse tracheal epithelial (MTE) cell monolayers grown on permeable filter supports in primary culture.
Although ultramafic host a number of endemic taxonomic entities that could be evolutionarily derived from closely related taxa from adjacent environments, so-called "neo-endemics" (Van der Ent et al. 2014, 2015), we could not detect any consistent morphological differences between the C. scortechinii from the Sabah ultramafic sites and that from elsewhere.
We found that the HA proteins derived from closely related human-derived H7N9 strains contain fewer T cell epitopes than other recently circulating strains of influenza, and that conservation of T cell epitopes with other strains of influenza was very limited.
Although most robust when sample RNA is derived from closely related cichlids, expression profiling results showed consistent hybridization for other closely related taxa (~65 million years divergence) and, to a lesser extent, even very distantly related species.
Although most robust when target RNA is derived from closely related species (<10 MA divergence time), our results showed consistent profiles for other closely related taxa (~65 MA divergence time) and, to a lesser extent, even very distantly related species (>200 MA divergence time).
We also calculated percent identity from MCALIGN2 alignments using insertion, deletion, and substitution parameters derived from closely related strains of S. cerevisiae (see Materials and Methods).
Correct sequence assembly is confirmed by high sequence similarities to partially sequenced var genes derived from closely related strains.
One way around this complication is to use cells derived from closely related or HLA-matched individuals or even the patients themselves, using induced pluripotent stem cells (iPS).
In 1979, a novel lineage of avian-like A(H1N1) influenza virus, believed to have derived from closely related Eurasia avian influenza viruses, emerged in swine in Europe [30] and replaced the classical swine A(H1N1) virus in this region [31], [32], [33].
In general, hybrids derived from closely related parents had relatively low levels of heterosis.
