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Our results demonstrate that hiPSC, regardless of how they were derived, can differentiate into a spectrum of rostrocaudal neurons with functionality, which supports the considerable value of hiPSC for study and treatment of patient-specific neural disorders.
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Taken together, these results indicate that the enhancing effects of miR-93 and miR-106b on reprogramming do not alter differentiation capacity of induced pluripotent cells and that those derived clones can differentiate into all three germ lines.
These results indicate that DFOG cells derived from GCs can differentiate into osteoblasts in vitro and in vivo.
The EPSCs derived from these protocols can differentiate into all embryonic and extra-embryonic lineages when implanted into chimeras.
Reh's current work offers more definitive evidence that immature cells, derived from muller glia, can differentiate again into mature neurons, says Michael Young of the Schepens Eye Research Institute.
Human mesenchymal stem cells derived from bone marrow can differentiate into osteocytes, adipocytes and chondrocytes, and maintain this multipotency during in vitro culture [14], [15].
Embryonic stem (ES) cells are pluripotent cells derived from blastocysts that can differentiate into almost all cell lineages in vivo [1] [3].
Neural crest stem cells derived from neural rosettes can differentiate into peripheral sympathetic and sensory neurons through the addition of BDNF, GDNF, NGF and dbcAMP, or into Schwann cells in the presence of CNTF, neuregulin 1β and dbcAMP [ 64].
Because uncommitted progenitor cells derived from adult blood can differentiate also toward the neural phenotype, it is possible that a broader derangement of immature circulating cells in diabetes predisposes to this chronic complication.
He then cultured them with two other cell types: endothelial cells, which make up the inner linings of blood vessels, that were derived from umbilical cord; and mesenchymal stem cells, derived from bone marrow, which can differentiate into several kinds of cells, though not as many as pluripotent cells.Cultures without mesenchymal stem cells failed to form a cluster.
Unlike WT-cell-derived RGs (which can differentiate into neurons, oligodendrocytes or astrocytes), the RGs derived from APPsw transgenic minipig formed only astrocytes upon directed differentiation.
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