We also compared our computationally derived localization assignments with assignments made using other computational methods with images from YGFP (Chen et al. 2007; Huh et al. 2009).
However, the automatically derived occlusion assignments may still be manually inspected and overruled, and an annotating user may sort occlusions by machine-given confidence values.
We benchmarked our computationally derived localization assignments for one of our wild-type screens, WT1 (Chong et al. 2015), to visually assigned localization annotations from YGFP (Huh et al. 2003) and found 94% agreement among the set of 1097 proteins assigned to a single location by both methods (Chong et al. 2015).
To identify the organisms from which metasecretome clones were derived, taxonomic assignments were designated for the predicted proteins of each insert, based on the best BLASTX hits, where the E-value was less than 1 × 10−5 and query coverage greater than 30%.
We gained general insights on the problem solution and derived simple assignment rules that lead to the global optimum by exploiting the dual problem formulation.
Also, it aims to derive optimal assignments between vehicles, drivers, and the destinations as well as an optimal selection and training of the selected drivers.
The loader is not fully compliant with the standard, but will import GO assignments and can be useful for loading assignments derived from the Blast2GO (33) tool.
In agreement with results from Urich et al. [23], our analyses indicate that taxonomic assignments based upon DNA 16S amplicons correspond well with assignments derived from ribosomal sequences within cDNA libraries.
On the basis of previous assignments derived from measurements of the WT and M109A derivatives, an H-site single-site variant is predicted to bind no CO at neutral pH but forms a CO complex at low pH where the S donor atom from M109 enters the coordination sphere.
For our research we have used a typical and important network with rudimentary functional assignments derived from a Saccharomyces cerevisiae PIN, as reported in [18].
Functional assignments derived from purely computational means were not considered.
