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Then, we derive lines that are maintained with reduced census size, and we analyze the consequences of inbreeding on the evolution of fitness and neutral genetic diversity.
In our particular case, employing this strategy would have reduced our expenditure by around 75%, a saving of approximately 700,000 USD, as our aim is to derive lines with HLA haplotypes that are the most frequent in any ethnicity.
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With federal money banned from being used in any part of the work on the derived lines, researchers had to divide their laboratories and go to extreme lengths to separate research materials based on the financing source.
Subsequently, four of the ex vivo derived lines were analysed for corresponding miRNA expression.
The current NIH guidelines allow research with newly derived lines, which Lamberth sees as inseparable from the destruction of embryos.
At least two independently derived lines on the autosomes were tested for each construct.
The artificially selected Agg line represents an extreme phenotypes beyond that seen in any of the naturally derived lines.
Several independently derived lines were examined for each fusion construct, and co-localization was quantified from two representative strains.
The change in absorbance at 340 nm per unit protein for ALBA22 and ALBA4 derived lines were expressed as a percentage of the line with the highest activity.
In this initial screen A22 and A4 derived lines were not significantly different from one another and were therefore considered as a single population.
Six of the ALBA22 derived lines with apparently single copy T-DNA integrations were subsequently selected and subjected to a more detailed analysis.
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