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The antibody derivatives were generated using a chain-shuffling approach based on human antibody variable gene phage-display libraries.
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In order to objectively evaluate chromosome territory (CT) positioning and to enable a statistical comparison between the parental cell line and its derivatives, 3D image reconstructions were generated using the software Image-Pro Plus (Figure 3).
The ΔNp63 α point mutant derivatives, C306R and C526W, were generated using QuikChange Site-Directed Mutagenesis Kit (Stratagene Inc., La Jolla, CA, USA).
Crossing point (CP) values were generated using 2nd derivative calculation software (LC480).
Deletion derivatives of pAD-WBLH1, pAD-WBLH2 and pBD-WBLH1 were generated using internal restriction enzyme recognition sites and internal primers with the linker sequence.
Sequence reactions were generated using transposon-specific sequence primers when pPDL2 derivatives were used as templates, whereas universal forward and reverse primers were used to generate sequence from subclones.
Random gene disruption strains were generated using a mini-Tn 10 transposon derivative having reduced bias in potential insertion sites [ 41].
Graphics were generated using R36.
Transgenic mice were generated using standard procedures.
The clusters were generated using Pearson correlation.
Figures were generated using SPDBV and PyMol.
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