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The antibacterial and antifungal activity of our derivatives was evaluated using the agar disc diffusion method and broth micro-dilution method.
The cancer preventive effect of aaptamine derivatives was evaluated using an anchorage-independent neoplastic transformation assay, as described previously [ 13].
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The wound repair properties of these derivatives were evaluated using in vitro and in vivo assays.
Partial derivatives are evaluated using finite difference approximations, but could also be implemented using the FFT [30].
In the outer core, radial derivatives are evaluated using combined compact finite differencing (Takahashi [2012]), whereas ordinary finite differencing is used in the D ″ layer.
Independently of their size, these approximate second-order Gaussian derivatives are evaluated using integral images, significantly speeding up the whole process.
The cytotoxicity of each active derivative was evaluated using MTT assay as described previously [ 28].
Their inhibitory activity against Aβ fibril formation was screened using ThT fluorescence assay, and the effect of derivatives on mitochondrial function was evaluated using JC-1 and MTT assay.
The efficacy of these derivatives to inhibit in vivo angiogenesis was evaluated using chick chorioallantoic membrane (CAM) model and their DNA cleavage abilities were evaluated after incubating with calf thymus DNA followed by gel electrophoresis.
The activity of the new derivatives against oxidative stress induced neuronal damage, was evaluated using glutamate-challenged hippocampal HT22 cells.
Binding was evaluated using flow cytometry.
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