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EREV8 is an EBV positive 293TetER derivative line generated by using cell-to-cell infection method [28].
In the present paper, using two lineage-related human melanoma cell lines, primary melanoma line IGR39 and its metastatic derivative line IGR37, two main observations are reported.
To confirm whether the expression of EBV Rta in 293TetER cells is sufficient to promote EBV lytic replication from the latent stage, rAkata-G418 EBV genome was transferred into 293TetER cells, yielding an EREV8 derivative line [28].
Comparison of the derivative line to the parental line identified a set of 54 differentially expressed genes.
To investigate the role of Bax in oxaliplatin-induced apoptosis, we utilised an isogenic HCT116 derivative line that differs only in the absence of a functional Bax gene.
Serial in vivo selection of a breast cancer cell line (MDA-MB-231) produced a derivative line (LM2) that metastasized readily to the lung in mice.
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cAt each backcross generation, genomic DNA was isolated from derivative lines that are triple heterozygotes for BB resistant gene linked markers.
Human breast cancer cell line MCF7 and its derivative lines BC19 (a gift from Julie Horton at National Institute of Environmental Health Sciences) and MCF7/AdVp3000 (a gift from Susan Bates at National Cancer Institute), HEK293/ABCC1 [14], HEK293/vector [14], HEK293/ABCG2 [4] were cultured as previously described [4], [13], [14], [22].
GL26 derivative lines (parental GL26, GL26nu, GL26B6, GL26B6V, and GL26B6-GFP) were validated by 100% intracranial tumorigenicity in T cell-deficient hosts at doses of 50,000 cells or less.
Promastigotes of L. donovani (MHOM/ET/67/HU3) and derivative lines LdMT knockout (ΔLdMT) and LdRos3 knockout (ΔLdRos3) [12] were maintained at 26°C in M-199 medium (Invitrogen, Karlsruhe, Germany) supplemented with 40 mM HEPES, 100 µM adenosine, 0.5 µg/ml hemin, 10 µM 6-biopterin and 10% heat inactivated fetal calf serum (Gibco, Invitrogen GmbH, Karlsruhe, Germany).
There are actually two major N2 derivative lines distinguishable by the presence or absence of a duplication of a portion of the left arm of chromosome V [ 17].
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