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Embryos with fewer than four cells had a significant advantage in derivation success compared to embryos with four cells on day two.
The likelihood of pregnancy is highest when the embryo on day two has exactly four blastomeres, but this parameter does not anticipate stem cell line derivation success.
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Our success in derivation of self-renewing oncospheres from pediatric high-grade brain tumors is comparable with or above the 50% average success rate reported to date on adult high-grade gliomas using the neurosphere assay [7], [9], [11], [13], [54], [55].
The large number (30) of permanent human embryonic stem cell (hESC) lines and additional 29 which did not continue growing, in our laboratory at Karolinska Institutet have given us a possibility to analyse the relationship between embryo morphology and the success of derivation of hESC lines.
This report describes the successful derivation and characterization of hESC lines from 2 out of 16 single blastomeres isolated from four top quality 4-cell stage human embryos (success rate of 12.5%).
The consideration of moderate strains whilst maintaining large rigid body movements is the key aspect for the success of the derivation.
Our success rate in derivation of permanent hESC lines was 12.8% (30/234) in 2001-2009.
This study represented the success of the derivation iPSCs in feeder- and serum-free systems.
Recent success in the derivation of haploid embryonic stem cells (haESCs) from mouse via parthenogenesis and androgenesis has enabled genetic screening in mammalian cells and generation of gene-modified animals.
These examples demonstrate that the success of stem cell derivation for any particular species may depend on the choice of the growth factors and/or culturing conditions.
The properties of these cell lines and clonal sublines with respect to their origins of derivation (primary versus metastatic lesions) and success or failure in transduction with the retroviral vector containing IFN-γ are listed in Table 1.
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