In the section on materials and methods (Sect. 6) we provide detailed derivations of the reported results.
Once basic commands are supported, an engine will be incorporated that orchestrates the execution of complex modeling workflows, involving simulation of trial designs, parameter estimation, derivation of inferences, and analysis reporting.
However, we should note that the derivation efficiency of stable TS-like cell lines in voles, under these conditions, is quite low whereas FGF4-assisted TS cell derivation was reported to be very efficient in mice [4], [12].
Here, by titrating exposure of HoxB4-ESC-HSC to Notch ligands, we report derivation of engineered HSCs that self-renew, repopulate multi-lineage hematopoiesis in primary and secondary engrafted mice, and endow adaptive immunity in immune-deficient recipients.
No earlier derivation of hESC has been reported from 3PN embryos.
To test this hypothesis, we report derivation of wild-type and PPARγ-null trophoblast stem (TS) cells.
Liao et al. (2009) reported derivation of rat iPS cells from primary ear fibroblasts and bone marrow cells by introduction of the four factors (OSMK).
However, there has been no success in establishing human TS cells, albeit a study has reported derivation of stable, proliferating cytotrophoblast stem cells from human ES cells [6].
All results for derivation of threshold values are reported on test data.
Additionally, SHEAR removed eight CREST predictions that were slight derivations of these four reported SVs and that SHEAR determined to be false positives due to sequencing error.
