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We start by giving a heuristic derivation of population models, distinguishing as usual between an activity-based and a voltage-based regime.
(A more detailed derivation of the model can be found in [27].) Note that there does not currently exist a complete, rigorous derivation of population rate-based models starting from detailed biophysical models of individual neurons, although some significant progress has been made in a series of papers by Buice and Chow on generalized activity equations for theta neurons [35 37].
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Despite these limitations, RDS was used to effectively recruit an urban Aboriginal population and allowed for the derivation of rigorous population estimates and data linkages that have previously not been possible.
This microscale approach to bacterial chemotaxis lends experimental support to theoretical derivations of population-scale transport parameters from single-cell behavior.
They use an arrayed cellular microenvironments (ACME) system to identify and optimize culture conditions to support derivation of this population of cells, and show that this MP population differentiates readily along the renal lineage, but does not generate blood or cardiac cell fates.
In the heuristic derivation of such population models it is usually assumed that each of the populations in the network is infinite, such that, in the spirit of the law of large numbers, the description of the activity in each population reduces to a description of the mean.
Due to the method of derivation of these populations (see Methods section), populations with same numerical subscript were treated as statistical blocks during analysis and block was always modeled as random factor.
How easy will it be to optimize the derivation of enriched populations of disease-relevant cells from human-induced pluripotent stem cells?
Following this strategy, i.e. derivation of cell populations from ROSA26-L-S-L-Luciferase transgenic mice followed by Cre-recombination in order to activate luciferase expression, autologous transplantation experiments can be performed in syngeneic luciferase-negative ROSA26-L-S-L-Luciferase transgenic mice with only the luciferase protein as potential immunogen.
The derivation of the analysis population is summarized in Table 2.
Furthermore, on a practical level, because not all tissue pieces are of sufficient quality for organ culture experiments we have found that tissue pieces with limited surface epithelium can still be put to good use in the derivation of primary cell populations [24], [42].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com