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Our data demonstrate these issues well, as there were discernible differences in the quality and depth of the sequencing reactions between cohorts A and C.
The coverage from bacterial and fungal samples was over 90%and98%8% respectively, indicating that the depth of the sequencing reads were sufficient for our analysis.
Although next-generation sequencing follows certain standard protocols, it can also be customized to address individual research needs, including factors such as the specific questions you are trying to answer, the depth of the sequencing that you require to achieve your research goals, and your budget.
It is commonly accepted that the depth of the sequencing can affect the results of an analysis [ 25– 28].
The average mapping depth of the sequencing data was approximately 14-fold, which is sufficient for accurate VB-based analysis.
Depending on the depth of the sequencing there is a different number of reads covering each CpG site (i.e. reads coverage).
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The time required to access the corresponding information measures the logical depth of the sequence.
This metric combines both the depth of the sequence alignments with the mean identity of the aligned reads.
Building upon this foundation, varDB will expand the breadth and depth of the sequence coverage as well as specific tools for the analysis of antigenic variation.
The size of the bin can be adapted to the depth of sequencing, the number of markers available and the size of the genome.
The number of similar sequences between different gene indices is dependent on the number of sequences available, the depth of sequencing from the various libraries, and the tissue diversity represented in the EST collections.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com