Sentence examples for depth of the cells from inspiring English sources
Twenty optical sections spaced 200 nm apart were taken through the depth of the cells using a 100× objective.
Z-stacks of 3 colors (Hoechst33342, GFP, and Alexa-Fluor 647) were acquired at 0.5 µm for the complete depth of the cells (approximately 19 20 Z-planes) and were deconvolved for 9 iterations with the appropriate (experimentally determined) point spread function (PSF).
When acquiring z-stacks, images were scanned at ∼120 nm intervals throughout the depth of the cells being imaged.
Image stacks were captured to cover the entire depth of the cells at a step size of 0.5 μm per slice.
Collection of a z-stack of confocal images (data not shown) showed this pattern to apply throughout the vertical depth of the cells.
Quantitative analysis of these mutations was performed allowing for reconstruction of the cells' lineage tree (Frumkin, D. et al., submitted), and for estimation of the depth of the cells (Wasserstrom, A. et al., submitted).
The density of the print at each point depends on the depth of the cell at that point and the quantity of ink it contains, rather than on the printing surface, as in the letterpress process.
The length was then multiplied by 0.6 cm, which was the depth of the cell and the contact area between oil and CO2.
Briefly, 300-nm optical sections were taken through the depth of the cell, and DeltaVision software (softWoRx) was used to deconvolve these images.
As previously shown [ 19] the low numerical aperture of the objective ensures that the phase measured is integrated over the entire depth of the cell.
