For NBT/BCIP staining, we identified a particular depth of staining with the substrate that allows comparison of different probes and that is suitable for OPT scanning and subsequent mapping of gene expression patterns (see [38], supplementary materials Fig. S1).
We observed a progressive depth of staining for M2 expression from normal to tumoral area (Fig. 1C ).
In order to standardise immunostaining runs, each independent observer categorised the depth of staining as dark, medium, or light, and the quality of the staining as granular or diffuse.
While A ρ and l c account for the variability in depth of staining and image size, the third parameter of the correlation function, D, uniquely quantifies the shape of the mass density spatial correlation function.
To account for the sample-to-sample variability in image contrast due to differences in the depth of staining, Bρ (r) was normalized to 1 at r = r min, where r min is the resolution of the image.
Determine the depth of the stain.
If the Kool-Aid stain has been dried on the carpet for quite some time, then this method may still work but depends on the age and depth of the stain and the type of carpet you have.
In an attempt to address issues regarding nonspecific staining effects and batch-to-batch variability, note was taken of degrees of immunoreactivity by categorisation of the depth of the staining as dark, medium, or light, and the quality of the staining as granular or diffuse.
Based on the clinical data, the best performing classification scheme involves a Decision Tree where the initial input has been stripped off from redundant features using the wrapper feature selection algorithm, maintaining the following features as most significant: depth of invasion, p16ink4a stain and N staging.
