It has seemed, therefore, that MPSS was the technology most likely to offer the depth of sampling required for whole transcriptome coverage, but this has not been adequately tested.
Recent advances in sequencing strategies make possible unprecedented depth and scale of sampling for molecular detection of microbial diversity.
First, the depth and breadth of sampling for oral microbiota have been insufficient in general, and the optimal parameters not determined for that of gingivitis patients in particular.
Considering depth of sampling there is clearly a confounding of depth and geographic location.
Mean depth of soil samples for measured soil variables was 30 cm with a range from 5 to 120 cm (Table 1).
Other less-significant uncertainty parameters were sampling methods, the variation in the soil field density with depth and the preparation of samples for measurement.
Database fields include information on sediment and biota samples (e.g., collection location and dates; sediment depth analyzed; average concentrations, variances, and number of samples for sediment and biota; organism lipid and sediment organic carbon content).
From the obtained results in Fig. 5 and the results from 6 further samples that looked qualitatively similar (not shown), we attempted to estimate the optimal sequencing depth in the following two ways using the available sequencing depth of the samples used for our analysis (TNBC samples: 34974017, 46677107, 17574408, and 24440340; TNBC-free samples: 25900791, 43454785, 31426867, and 33517581).
The PCR products were then sheared to approximately 500-bp fragments and used to construct libraries for sequencing using Illumina Hiseq4000, yielding 100-bp paired-end reads with average per site read depth of 1,757 for human samples and 1,368 for mouse samples.
For this reason, the effective sampling depth of CREL for broad beam while fiber to beam distance of 0 mm is about 2 mm larger than that of CR.
