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Different statistical methods of analysis were applied to investigate the spatio-cardinal distribution of PXD in soil in a pilot wheat field and to explore the individual and interactive impact of different parameters such as cardinal axis, sampling time after application, and depth of sampling site on PXD residue.
In addition to the deep reversing thermometers, unprotected reversing thermometers were applied at five levels to determine the depth of sampling.
In addition to providing useful comparisons of high-throughput technologies, variable regions and analysis protocols, this analysis acts as a pilot study for validating methodologies for a large-scale national metagenomics initiative (see http://eldermet.ucc.ie), by defining how much sequencing is necessary to sufficiently capture the community diversity at an affordable depth of sampling.
It should nevertheless be noted that prevalence measures are dependent on depth of sampling.
Here, we directly compared differences in treponeme OTU composition within periodontitis and periodontitis-free subjects using a similar depth of sampling.
In such cases, the use of next-generation sequencing technologies may offer practical advantages in terms of cost, throughput and depth of sampling.
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A simple model: Y = a [1-exp (−b/x)], was used to calculate the mean soil C stock within the depth of 100 cm in both plantations and natural forests, where x was the depth of sampled soil, Y was soil C stock, a and b were estimated parameters.
The depth of sampled wells ranged from 6 to 1,500 ft and had a median depth of about 140 ft. The term "sample" represents a distinct geographic site and applies to an environmental domestic well sample collected at the wellhead before household treatment.
We therefore concentrated our sampling on the topmost gardens, which we generally encountered at depths of 30 60 cm (depths of sampled gardens are listed in column D of Table S3).
Recent advances in sequencing strategies make possible unprecedented depth and scale of sampling for molecular detection of microbial diversity.
Tone darkens with depth of sample, as shown in legend.
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