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SHG images of the dermis were acquired using the excitation wavelength of 900 nm and a power in the 20 mW–60 mW range, depending on the depth of recording.
TPF images of the epidermis were acquired using the excitation wavelength of 740 nm, which is adequate for exciting NADH fluorescence, and a power in the 10 mW–40 mW range, depending on the depth of recording.
Two excitation wavelengths were used: 740 nm for exciting NADH, and 890 nm for FAD, and a mean laser power varying between 10 and 40 mW, depending on the depth of recording.
We examined the relationship between the depth of recording sites and cortical layers after extracellular recording sessions were completed for all the spots (Fig. 4 B and Table 1).
TPF images in the epidermis were typically acquired using the 40x objective lens, 740 nm as excitation wavelength, a resolution of 512 × 512 pixels (corresponding to a field of view of about 200 μm), using a pixel dwell time of 20 μs, and a power in the 20 mW–50 mW range, depending on the depth of recording.
SHG images in the dermis were typically acquired using the 20x objective lens, 900 nm as excitation wavelength, a resolution of 512 × 512 pixels (corresponding to a field of view of about 400 μm), using a pixel dwell time of 20 μs, and a power in the 30 mW–60 mW range, depending on the depth of recording.
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Furthermore, the single-item measure 57 may compromise breadth and depth of recorded data for speed of administration.
The 'in-depth' method of recording children's journeys enabled good quality data to be collected, which was the strength of this study.
The resting un-normalized log alpha peak power was divided according to the depth of each recording site into subregions spanning 4 mm along a rostrocaudal axis.
The tool head of IProMS can be located inside a pressure tube at any axial location under in situ condition and the properties can be estimated from an analysis of the data on load and depth of indentation, recorded during the test.
An interesting finding in the single-unit horizontal connectivity analysis was that the connectivity between nearby units decreased as the depth of the recorded neurons increased [25].
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