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The depth of cell infiltration into the acellular tissue decreased with increase in cross-linking degree.
Generally, the depth of cell infiltration into the acellular tissue decreased with increasing its crosslinking degree.
Z-sections of confocal images revealed that the depth of cell growth inside scaffolds was consistent with the order of mesh size.
Z-stacks were collated on a confocal laser scanning microscope to determine the depth of cell migration into the gel.
Particularly, there is a lack of methods that can quantitatively assess the degree ("depth") of cell senescence.
The depth of cell invasion was determined by measuring the distance from the top of the gel to the leading front of migrating cells.
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We did not present analysis results that use this measure since we found them to reflect mostly on the depth of cells.
The large image shows a merged image of 6 Z-Stack confocal images (1 μm depth) of cells with microtubules (in red) and GFP-BtpA in one of the cells.
Caco-2 cells were labelled with fluorescence-labelled BoNT/A throughout the depth of cells and showed the typical ringed labelling where there is nuclei exclusion and presence of toxin-labelled vesicles (Supporting Information Figs S2 and S4).
Stacks of images were collected throughout the depth of cells, using a Zeiss LSM510 confocal microscope (Carl Zeiss MicroImaging, Jena, Germany) with a x63NA1.4 oil immersion objective using a 405-nm laser for DAPI and a 561-nm laser for Alexa-568.
These results hence provide direct evidence that the mechanotransduction at different depths of cell body is mediated by differential sets of mechanosensing elements.
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