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Essentially, the optical sectioning picture breaks down when approaching the fundamental depth limit.
Its depth limit is the somewhat arbitrary boundary between soil and "not soil" (e.g., bedrock).
Onstott, T. C. et al. Does aspartic acid racemization constrain the depth limit of the subsurface biosphere?
The depth limit achieved in this study is low compared to conventional spinning.
"Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging", Biomed.
First, there is no depth limit for detection of fractures with flow.
Jang, M., Ruan, H., Judkewitz, B. & Yang, C. Model for estimating the penetration depth limit of the time-reversed ultrasonically encoded optical focusing technique.
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Theer, P. & Denk, W. On the fundamental imaging-depth limit in two-photon microscopy.
We are interested in breaking and extending such a fundamental imaging-depth limit for ultra-deep tissue imaging.
"Extending the fundamental imaging-depth limit of multi-photon microscopy by imaging with photo-activatable fluorophores", Opt.
Quantitatively, the depth where the in-focus signal and the out-of-focus background are equal to each other is defined as the fundamental imaging-depth limit.
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