Briefly, cells were washed with warmed PBS and OGD solutions, and covered with oxygen deprived OGD solution.
After one week, half of the plants were transferred to fresh solution containing 250 μM Pi, while the other half was transferred to nutrient solution deprived of phosphate (Pi), with H2PO4 being replaced by H2SO4 [ 127].
The rock-wool was rinsed thoroughly with tap water to remove nutrients, before adding nutrient solution deprived of nitrogen (referred to as day 0).
Briefly, seedlings were transferred into a hydroponic system after 3 days of germination and grown for further 14 days in a solution deprived of nitrogen.
The seedlings were grown in a control complete nutrient solution (1) and in a control N-deprived solution (3) for 14 days.
Results showed that combining vernacular passive strategies with affordable active renewables such as roof top solar panels results in a hybrid energy efficient retrofitting solution for deprived off-grid vernacular buildings.
Adult female C. pipiens used in the host selection trials were at least 4 days post-emergence and were deprived from the sucrose solution for 24 hr prior to each trial.
To induce P deficiency (−P) the nutrient solution was deprived of K2HPO4.
Subsequently, the plant nutrient solution was deprived of Me-JA and the PvTIFY10C transcript level recovered, after 12 h, to almost basal levels.
I could have solved her problem easily by giving her the answer, but that would have deprived her from finding the solution herself.
The need to deploy water solutions quickly to deprived areas is unlikely to recede anytime soon.
