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An analysis of body weights from the P2+P7 PCP experiment showed that across postnatal days P2 P14, the PCP-treated mice weighed significantly less than either the normal (suckle) or deprived control groups (p<0.0005) (Fig. 3A).
In addition, the deprived control mice weighed significantly less than the normal suckle control group yet no differences were found between these two groups on any of the behavioral measures.
Analyses of the normal and deprived control groups again showed no significant differences on any learning and memory measures at any age (P30, P75, P170) so the data from the two groups were combined for statistical analyses conducted on the P2+P7 PCP-treated mice in an effort to simplify the interpretation of the effect of PCP treatment on learning and memory.
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A clear trend with deprivation was evident: the average radon concentration for the most deprived controls being 16.1 Bq m−3, compared with the least deprived of 27.2 Bq m−3.
Animals treated with a single dose of PCP on P2 weighed significantly less than either normal (suckle) (p = 0.009) or deprived controls (p = 0.00001) across postnatal days P2-P14.
Exposure to a single dose of PCP on P7 also resulted in decreased body weights across postnatal days P7 14 compared to deprived controls (p = 0.00009) and at P21 [F 1,28) = 11.974, p = 0.0017). Body weight analysis at P45 revealed no significant differences between groups.
Preliminary analyses were conducted between the normal and deprived controls with regard to the data from the cued, place and probe conditions in the water maze and from the conditioned fear task, with the result being that no differences were observed between the two groups on any of the learning and memory measures.
We found that the amplitude of the mean uEPSP1 was not correlated with the mean uIPSP1 amplitude in either control or deprived cortex (control, r = −0.06, n = 17 reciprocally connected FS → Pyr pairs, P = 0.796, Pearson correlation; deprived, r = −0.40, n = 14 reciprocally connected FS → Pyr pairs; P = 0.159, Pearson Correlation) (Fig. 7 F).
Indeed, MD induced a significant decrease of the D/M ratio in deprived versus control rats (Fig. 6a; MD 11.48%±1.16 versus control 18.31%±1.62; t test, P = 0.003, N = 10) in the hemisphere contralateral to the deprived eye.
Accordingly, the D/F ratio was significantly higher in deprived versus control animals in both hemispheres (Fig. 5b; contra, MD 1.72%±0.21 versus control 1.09%±0.06; t test, P = 0.017; ipsi, MD 2.03%±0.36 versus control 1.09%±0.17; t test, P = 0.041, N = 11), indicating that the long-term deprived eye retained its ability to activate cortical PV neurons.
The gibel carp showed no difference in gross growth efficiency between deprived and control fish.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com