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This may result in the deployment of linker histones with a less dynamic range of binding affinities expected from the short OdH1.1, OdH1.3 and OdH1.4 proteins.
The regulated deployment of NETs may require covalent modification of histones, the basic DNA-binding proteins that organize chromatin in the cell's nucleus and within NETs.
"Two features of histone modifications are notable," Allis said.
There are five classes of histone.
"We became interested in the process of histone modification," he said.
"The genes that were turned 'on' were invariably associated with one form of histone, while the genes that were turned 'off' were invariably associated with a different form of histone".
The homeostatic balance of nucleosomal histone acetylation is maintained by actions of histone acetyltransferases and histone deacetylases (HDAC).
Two classes of histone lysine demethylases remove histone methylation.
Though we pick up histone H3 in the present experiment, the roles of other histones, especially the role of histone H4, should be examined in the future study.
Nucleosomes reconstituted from bacterially expressed histones are useful for functional and structural analyses of histone variants, histone mutants, and histone post-translational modifications.
Table 1 O-GlcNAcylation sites and functions of histone tails.
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