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The depletion procedure used in the third protocol of sample preparation allowed us to estimate inter-individual variation of von Willebrand factor concentration (1.15 fM, CV = 59%%) in six samples.
A capillary depletion procedure [58], [59] was employed to separate the cerebral capillaries from the brain parenchyma.
Moreover, the protocol suggested by the supplier of ProteoPrep20 indicates, as an optional step, the precipitation of proteins prior to trypsin digestion and MS analysis (i.e. after the depletion procedure).
(D ) Left : schematic of the biotinylated TERT depletion procedure.
(F ) Left : schematic of the biotinylated TERT depletion procedure and unbound fraction analysis.
The depletion procedure was repeated once more with freshly prepared anti-LTA-Sepharose beads.
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In our original experience, many samples generated using RiboMinus depletion contain up to ∼50% rRNA reads, the more recent rRNA depletion procedures are much more efficient (see above) and thus more cost effective.
All subjects were scanned in the absence of pharmacological intervention (baseline) and after two different depletion procedures.
Common AMPT depletion procedures involve a 25- to 48-h study period in which study subjects administer a total amount of 4,500 8,000 mg AMPT [ 6].
Concerning the isolation of Chlamydomonas lipid mutants, the effect of salt addition provides a convenient alternative to N-depletion procedures for inducing oil accumulation in mutant screens, which are often performed in microplates.
The depletion scoring procedure considers all footprint segments identified by the DBN.
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