Exact(1)
There are limitations to relying on an egg-based manufacturing system including egg allergies in a small percentage of the population as well as potential issues with egg supply for surge capacity or potential depletion of egg supply due to avian influenza outbreaks.
Similar(57)
Finally, experiments with a chemotherapy drug called busulfan, known to selectively kill off sperm stem cells in men, likewise appeared to obliterate egg replacement in mice without killing existing eggs, leading to severe depletion of their egg stock over time.
Depletion of HFG from eggs, following the feeding of an HFG-free diet was 2.6 days, somewhat slower than the corresponding values for lasalocid and nicarbazin (1.1 and 1.6 days, respectively).
Finally, the depletion of ciprofloxacin in both egg compartments and its effect on biomolecules were evaluated.
After depletion of endogenous Eg5 from egg extract and addition of mutated Eg5 deficient for phosphorylation by Cdk1, we found that this mutant localized to microtubules in amounts that are far below the critical concentration that is required for bipolar spindle formation, unless added at unnaturally elevated concentrations.
It was noted that depletion of TPX2 from Xenopus egg extracts results in the formation of less compact spindles [ 50].
Depletion of Gwl from Xenopus egg extracts impaired checkpoint recovery, as judged by persistent activation of checkpoint proteins and delayed reactivation of Cdk1 after removal of DNA damage.
Depletion of CPEB from an egg extract abolishes polyadenylation and injection of a CPEB antibody blocks it in oocytes and embryos [20,21,31,32].
Depletion of Crk II from egg extracts prevented apoptosis, and these studies further demonstrated that Crk binds to the cell cycle regulatory protein Wee1, implying that this pro-apoptotic function of Crk II may be due to nuclear localization [ 206].
However, depletion of ATR from the egg extract did not affect the induction of ATM activation by single- and double-stranded DNA molecules, indicating that ATM was directly activated by these DNA structures (Supplementary Figure 3A and B).
On the other hand, in Caenorhabditis elegans, disruption of the NIPBL ortholog does not alter the binding pattern of neither condensin nor Smc5/6 (Lightfoot et al. 2011), and depletion of Scc2/4 in Xenopus egg extracts clearly affects cohesin but not condensin loading (Gillespie and Hirano 2004).
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