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Because we have not used an alternative method of measuring mitochondrial content (e.g., electron microscopy), we cannot say whether the depletion of complexes III and IV occurred in isolation or as part of mitochondrial depletion.
The ability of aerobic ATP synthesis (OXPHOS) was reduced to ~54%% in ICU patients (p<0.01), in correlation with the depletion of complexes III (~38 % of control, p = 0.02) and IV (~26%% of controls, p<0.01) and without signs of mitochondrial uncoupling.
The resultant loss of polyadenylation causes a differential modulation of steady-state levels of specific mt-mRNAs and perturbs mitochondrial protein synthesis leading to profound depletion of complexes I and IV of the respiratory chain, resulting in a form of spastic ataxia and optic atrophy.
Similar(57)
We found a depletion of complex III and IV concentrations and relative increases in functional capacities of complex II and glycerol-3-phosphate dehydrogenase/complex III.
In Madin-Darby canine kidney (MDCK) cells, siRNA-mediated reduction of DPAGT1 expression by only 40% results in a striking depletion of complex N-glycan modification of E-cadherin (Nita-Lazar et al. 2010).
Simultaneous depletion of both complexes causes the severest defect.
It would seem that some more experiments on the biological effects of depletion of the complexes should be included.
Failure of A-Complex recruitment due to mutation in the BRCT motif, or depletion of A-Complex components including BRCC36/45 (deubiquitinylases) would alter the chromatin ubiquitinylation pattern.
The operation of all these soil processes regulated by B. aryabhattai strains resulted in a depletion of organically complexed and calcium carbonate bound zinc and an increase in exchangeable and sesquioxide bound zinc in soil.
Upon severe depletion of this complex, chromosomes fail to congress successfully and progression through mitosis is compromised.
Accordingly, depletion of respiratory complex proteins has been described in the diaphragm in a rat model of sepsis [ 20].
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