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Depletion efficiency was equally efficient in single and double depletions.
The procedure applies both hypotonic lysis to remove red blood cells (RBCs) and anti-CD45 antibody to deplete WBCs at a depletion efficiency range of 2 3 logs [12, 29].
We noted that these differences in cbp-20 and cbp-80 depleted animals occurred although relative depletion efficiency was comparable for both mRNAs (Supplementary Figure S4A), possibly indicating that differences in protein stability or differences in protein abundance already before depletion might render CBP-20 more refractory to efficient depletion by RNAi.
Depletion efficiency in the spleen and tumor of IL-15 TG/MT mice when depleted with anti-NK1.1 (A) or anti-CD8α (B/C) antibody long term starting at 4 weeks of age (representative flow plots from n = 3 in each).
The depth dependence of resolution is found to be consistent with the depth dependence of depletion efficiency, suggesting resolution is limited by STED laser propagation through turbid tissue.
The depletion efficiency and the concentration of Eg5 after add back were determined by Western Blot from 0.5 µl extract loaded onto a SDS gel.
Similar(33)
Each time point was also compared to non-arrested cells to assess expression and depletion efficiencies.
For the Asf1a shRNA, we also designed an miRNA-based loop in the shRNA because it was reported to result in better depletion efficiencies [85].
For qPCR analysis of siRNA-mediated depletion efficiencies (Fig. 3A) RCK and TNRC6A mRNA levels were normalised to mRNA levels of the ribosomal protein RPL13a.
Depletion efficiencies were assessed using a Bioanalyzer platform (Agilent).
Thus it would help to have data/comments on the depletion efficiencies.
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