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The anti-ASGM1 antibody depletion achieved greater than 50% depletion of NK cells over a 49 day period as confirmed by monoclonal anti-CD3 and anti-NK1.1 antibody (BD Bioscience) staining and flow cytometric analysis.
The extent of LERP depletion achieved in these experiments amounted to 72±1%1% (Fig. 5A).
The median CD8+ cell depletion achieved was 93% (range: 84%-98%).
In addition, polyamine depletion achieved after treatment with DFMO gave the opposite effect.
The profound T cell depletion achieved with Alemtuzumab in the periphery and lymphoid tissues, prompted us to examine whether it also reduces endomysial T cells.
Factors influencing antigen and effector modulation are thought to be important in determining the final extent of depletion achieved (Table 1) [ 18, 27, 28].
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Nitrogen depletion was achieved as harvesting and transferring cells to nitrogen depletion medium.
Macrophage depletion was achieved by treating nude mice with liposomal clodronate, which induced 80%-90 80%-90tion of macrophages monitored by testing for the peritoneal macrophages and spleen macrophages (data not shown).
T cell depletion was achieved following IP injection of 150 μg of depleting antibody into female age-matched FVB mice for 3 consecutive days and was maintained by further IP injection at 3 day intervals until the study was terminated.
In these studies, Kupffer cell depletion was achieved by clodronate liposomes injection, a technique that efficiently depletes all resident macrophages from liver and spleen (van Rooijen 1989; van Rooijen and Sanders 1997).
Nitrogen depletion was achieved within 24 h and 2.75 g/l of cell concentration was achieved (Figure4c and d).
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