Sentence examples for depleting amount from inspiring English sources
Lysates were cleared at 20,000× g for 30 min. For IP, lysates were diluted to 1 mg/ml in RIP buffer (20 mM Tris pH 7.94°C, 200 mM KCl, 0.05% IGEPAL CA-630, 10 mM EDTA), cleared by centrifugation at 20,000× g for 10 min, and incubated with a depleting amount of antibody (predetermined with titration experiments) for 3 hr at 4°C.
The number of reactions for each type of kit reflects the depleted amount of rRNA in prokaryotic or eukaryotic samples.
The ozone layer of the Earth's atmosphere plays an important role in depleting the amount of ultraviolet (UV) radiation that reaches the surface.
Therefore, we hypothesized that L1 RNA and intronic pre-mRNA of genes containing L1s, because of complementarity of both sequences, form dsRNA which may be targeted by RISC, consequently depleting the amount of mRNA derived from genes containing L1s.
If the water in the pond is insufficient, it is then necessary to pump water from an existing nearby dam, thus depleting the amount of water that has been stored in the dam.
Our consumption of energy has been steadily growing, the costs are increasing, and in the process we are depleting vast amounts of fossil fuel reserves and building up dangerous levels of carbon gases and pollutants.
Then, when total RNA input was > or equal to 1 μg, depleted RNA amount was checked by Qubit RNA HS Assay quantification and 40 ng, or less, were used to synthetize cDNA with SMARTer Stranded RNA-Seq Kit (Method ID: RiboZero_SMART_Strand).
These blooms deplete the amount of oxygen in the water, threatening aquatic life.
Privately drilled borewells have quickly depleted the amount of groundwater in the city.
At the current sales pace, it would take 1.32 months for distributors to deplete the amount of goods on hand, compared with 1.31 months in February.
Interestingly, we show that Nef depletes the amount of MHC-I in Rab11 positive recycling endosomes and that a functional early endosomal compartment is required for Nef-dependent MHC-I downregulation.
