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Our key conclusion (that Kog1 bodies increase the threshold for TORC1 activation) depends on experiments we did comparing the level of Sch9 phosphorylation in strains carrying mutations in TORC1 to the level of Sch9 phosphorylation in the wild-type strain.
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Tensions around time frame are especially salient, because a firm's long-term survival depends on experimenting, taking risks, and learning from failure in the pursuit of new products, services, and processes.
Mainly depending on experiments and tests, traditional research in materials science is costly and time-consuming.
It is related primarily with the fluid-solid interaction, whose studies mainly depend on experiments at the present time.
Depending on experiments, cells were plated on 1 µm pore size microporous PET filters (Falcon, BD Biosciences, Franklin lakes, NJ), or in plastic flasks (Falcon) or on glass lamellae (Polylabo, Strasbourg, France).
For all the purified proteins analysed, 100 μl of protein sample (1 15 nmol depending on experiments) were injected on to the column.
Thymocytes, 2 × 10 cells/ml, were incubated in RPMI 1640 medium at 37°C, 5% CO2 atmosphere with 1 µM dexamethasone for various time points depending on experiments.
The pulling movement of the machine was represented by a vertical displacement equal to 1.4 mm (depending on experiment) applied on the upper pin.
Depending on experiment, single cell suspension contained either 2.5×104 fibroblasts / ml or 5×104 SCC cells / ml.
For SEPT9 measurements on samples, reactions were performed on 10 12.5 µL of undiluted DNA depending on experiment.
However, the immunoblot signals for GnK48 and GcW1 alleles were lower (∼25 50%, depending on experiment) than for their wild-type counterparts making it necessary to normalize for input.
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