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To aid in the cloning of ADE2 between the loxP sites and the addition of the NES element, destroy the 3' Kpn I restriction site and replace with an Xho I restriction site (Note: depending on your sequence, it might be necessary to use a different restriction site).
To aid in the cloning of ADE2 between the loxP sites and the addition of the NES element, destroy the 3' KpnI restriction site and replace with an XhoI restriction site (Note: depending on your sequence, it might be necessary to use a different restriction site).
Similar(58)
With increasing sequencing depth, error rate substantially decreases, but some errors remain and the error rates may depend on sequencing cycles, sequence context and other factors (45, 46).
This step is important, as the interpretation of one-letter sequences will vary depending on the sequence type.
As he put it in a letter to his friend Richard Curle: "my 'art'... is fluid, depending on grouping (sequence) which shifts, and on changing lights giving varied effects of perspective".
And so, when you do the reverse engineering, it could be a couple of different locations, depending on the sequence of which these temperature sensors were either lost or the heating took place.
Linear lncRNAs can act as a scaffold to recruit proteins depending on their sequence or structure.
These threats are explained depending on the sequence of the steps of the experiment.
Another concern was the different scores depending on the sequence of biomarkers owing to tissue heterogeneity.
Interestingly, EDM1 contains few possibilities of formation of CpG dinucleotides depending on the sequence, from 0 to 3 CpG.
Furthermore, depending on the sequence specificity of MOs, MOs may have caused secondary effects like p53-dependent apoptosis [31].
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