However in our hands with the H441 cell line where with no CFTR present we found that there was no significant change in NF-κB activity when treated with the inhibitor, however when CFTR was present there was a significant increase in NF-κB activity, suggesting that the effects of the inhibitor may differ between cell lines, depending on which pathways remain intact (compare figure 3C with 1D).
The overall response of neurons to stimuli, however, depends on which pathways are predominantly regulated.
A prediction of this model is that in these rare lineages, the spectrum of mutations will be biased, revealing which repair pathway is defective, and the bias will be different in different lineages depending on which repair pathway is/was impaired.
We suggest that oligomycin can have different effects on ATP levels in different cell lines, depending on which metabolic pathway (glycolysis vs. oxidative phosphorylation) is mainly active.
Depending on which metabolic pathway predominates at critical junctures of exposure and tissue susceptibility, fast acetylators may be at higher or lower risk of smoking-induced breast carcinogenesis than slow acetylators.
Thus, mGluR5 can produce either excitotoxicity or neuroprotection depending on which cell signalling pathway is activated.
Antiphosphotyrosine hybridization results highlighted the progressive loss of phosphoproteins during time course with individual variability probably depending on which specific phosphoprotein pathways become activated or suppressed ex vivo.
After DNA damage induced by IR, cells enter either cell-cycle arrest or apoptosis, depending on which of the pathways is predominant in the specific cell type and environment.
The observation of different effects in Giardia depending on which gene in the RNAi pathway is knocked down, leads us to believe that some proteins in the pathway have roles in the cell beyond that of just RNAi.
The next step in our hierarchical program, however, would be to take the next decay patterns and fit them to the network given in Figure 5 activating the appropriate pathway depending on which factor (NOT1 and/or PAN3) is present while keeping λ R fixed.
