Sentence examples for depending on template from inspiring English sources

Then, depending on template source, 40 or 45 cycles of 95°C for 30 seconds, 55°C for 1 min and 72°C for 1 min followed and the reaction ended in a final extension of 10 min at 72°C.

CCS allows for the repeated sequencing of individual inserts and, depending on template length, stochastic sequencing errors are reduced with each CCS pass [ 16, 17].

Various combinations of the used primers are presented in Table  5.> For cox1 and ITS, 25 μL PCR mixtures were set up with 0.5 U Taq polymerase (Qiagen), 5 μL 10x buffer, 2 4 μL MgCl2 (depending on template and primers), 0.8 μL dNTPs, 0.5 μL from each primer (20 ng/μL).

Amplicons up to 5 kb were amplified using the GC-Rich PCR System from ROCHE, with the following modification to the method used for cDNA synthesis: 400 ng of genomic DNA as template; for the first 10 cycles elongation of 5 to 8 minutes (depending on template size) at 66°C temperature, then increase of 5 s for each successive cycle; final elongation at 66°C for 7 minutes.

An error of a measurement depends on template subset size, search region size texture quality.

In generic programming, it is often difficult or impossible to express types that depend on template parameters, in particular the return type of function template instantiations.

Its primary intended use is in generic programming, where it is often difficult, or even impossible, to express types that depend on template parameters.

First, it depends on template DNA quality.

However, the results obtained with this test also depend on template input and setting the reading threshold for obtaining Ct values, two parameters needing further standardization.

Variations in RAPD patterns are chiefly attributed to variations in the priming efficiency during early rounds of amplification, and these in turn depend on template concentration and purity, primer/template ratio, or the ramp times of the cyclers used.

Distinct morphologies result depending on the template pre-treatment, synthesis temperature, template dimension and the relative polarity of the porogenic solvent.