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Depending on staining intensity, 5 to 8 μL of sample were injected into LC-MS/MS system.
Sections were then stained with Fast Red (Sigma FastTM, F-4648, St . Louis MO) for 10 15 minutes depending on staining intensity.
Strength of CD20 reactivity was subjectively scaled from 1+ to 4+ depending on staining intensity, thus intensity ≤2+ was regarded as weak and intensity>2+ was regarded as strong).
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In these studies, visualization of the blood- and lymphatic vasculature depended on staining by in situ hybridization (ISH).
However, as the absolute dye signal depends on the amount of staining one divides the raw signal by F0, xy.
This showed that a variable amount of spot fall-out was seen after staining of the frozen sections, depending on the staining method, indicating that the choice of staining method can be important in minimizing loss of mRNAs from microarray analysis of LCM-derived RNA.
The staining results for EGFR and HER-4 expression, in which positive staining is defined as any staining of tumour cell membranes above background level, whether it is complete or incomplete circumferential staining, have been classified into three levels (+, ++, and +++) depending on the staining intensity.
Stained TMA slides were graded jointly by two pathologists using a visual semiquantification method (optical density of the immunoreactivity) depending on the staining characteristics of the antibody.
Depending on the staining method applied, cell biomasses yielded 15 86% of the biomass defined by the model, and good correlations between cell biomass and model biomass were found.
Depending on the staining procedure, either a stereomicroscope (Leica M, Leica IM 50, Solms, Germany) or a confocal microscope (Leica DMR-E, Nussloch, Germany) was used for documentation of representative digital images shown in this article.
Colonies were then scored depending on their staining.
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