10.7554/eLife.00290.019 Figure 7. Metaphase spindle orientation in stage 3 embryos depends on spindle size.
T. brucei undergoes a closed mitosis and forms a mitotic spindle within the nucleus (Ogbadoyi et al., 2000), and segregation of both megabase chromosomes and minichromosomes depends on spindle microtubules (Ersfeld and Gull, 1997).
DOI: http://dx.doi.org/10.7554/eLife.03842.013 BB positioning and stereocilia bundle morphogenesis depend on spindle positioning proteins (Ezan et al., 2013; Tarchini et al., 2013), but how these connect to core PCP molecules and actin-rich stereocilia bundles is unknown.
Using mechanical micromanipulation to remodel the spindle, we showed that spindle microtubules could reassemble into lateral or asymmetric spindles, depending on whether the spindle was remodeled by collapsing or by folding.
In potato, 2 n pollen depending on parallel spindles was described long time ago but the responsible gene has never been isolated.
Symmetric cell divisions underlie tissue spreading or directional tissue morphogenesis, depending on whether the spindle is randomly oriented within the epithelial plane (z axis) or assumes a fixed planar orientation.
The suppression of chromosome missegregation in CUP-CLB3 ndcells cells did not depend on the spindle assembly checkpoint, because deletion of MAD3 had no discernable effect on the extent of ndc80-1 mediated suppression, nor was it due to the ndc80-1 aloweringwering Clb3-CDK activity.
Metaphase plate position may also play a crucial role in asymmetric cell divisions that depend on asymmetric spindles in anaphase, such as in embryonic D. melanogaster neuroblasts.
Depending on the length of the spindle, ∼2 to 6 pixels were averaged per length bin.
Microscopically the tumors were categorized as spindle or epithelioid depending on the most represented cell type (> 50%) throughout the tumor.
